@snorwood, this looks like you have the local absolute path to your conditions file rather than the relative path (i.e. relative to your PsychoPy experiment file). If you go to the loop, where you have your conditions file set up, make sure the path is just “Conditions_1B.xlsx”. In addition, make sure that “Conditions_1B.xlsx” is in the same directory as your psyexp file, so the relative path of the conditions file is correct.