Search Array with Cues

Hi, this is my first time using PsychoPy for my thesis project as a college senior. We are conducting a visual search experiment in which certain cues appear at a fixation point and then a search array will appear in one of the four quadrants of the screen. Each cue image file fits with a few search arrays. How do I portray this in my excel file? I need to list all 500 search files in the excel and their location. Do I also need to portray that there is a cue that matches with each search file within this excel sheet? I want it to consist of a loop of about 50 trials with a break in between each one. How to I get sampling without replacement to occur for these loops? Thank you so much!