Hi @SaraCal,
It seems like passing in the names of ‘cue1’ and ‘cue2’ when setting opacity for the rest of your stimuli inside of ‘fixation_cueRoutineBegin’ is causing the error.
Could you please try passing in numeric values instead? If your intention is for eg. ‘cue1a_main’ to have the same opacity as ‘cue1’ as a result, you should be able to just use ‘cue1.opacity’ I believe.
I hope that solves the problem, here to help if not.