Hi Mara,
“Portmidi closed” is not an error message but only a warning. It only means that there is no MIDI interface connected to the system at the moment the audio server is booted. Totally harmless…
I notice two things in your setup:
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A buffersize of 32 samples is unlikely to work well under windows, I think you should increase it to somthing like 128 or 256.
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In your preferences, sounddevice comes before pyo… Are you sure pyo is always used in your experiment? I tough that psychopy try the audio backend in the order given in the list.